PCE| CsTFL1和CsTFLd响应温度和光周期信号，以抑制黄瓜测定的生长
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植物通过优化花序结构来监测环境因素以平衡其营养生长和生产生长。顶花1（TFL1）及其同源基因调控黄瓜的花序结构，但其对环境因子的反应机制和顶花的形成尚不清楚。在这里，我们进行了基于图位的克隆，以确定控制季节依赖性生长表型的基因，并发现它是由黄瓜系WI1983Hde基因组中CsTFL1的完全缺失引起的。在CsTFL1缺失植株（CsTFL1del）中，高温和长日照条件可以部分地挽救确定性生长。
长日照和高温信号可上调CsTFL1及其同源基因CsTFL1d的表达。CsTFL1d基因敲除可导致野生型小鼠的生长和终末花的形成，表明长日照和高温诱导CsTFL1d表达可能部分挽救了CsTFL1del的生长。
此外，生化分析显示CsTFL1d与CsNOT2a直接相互作用，表明CsTFL1d和CsTFL1通过相似的调控机制发挥作用。我们的数据表明CsTFL1和CsTFL1d通过响应温度和光周期信号共同抑制确定性生长。它提供了关于环境线索如何塑造黄瓜花序结构的机制性见解。
Plants monitor environmental cues to balance their vegetative and productive growth by optimizing their inflorescence architecture. TERMINAL FLOWER 1 (TFL1) and its orthologs regulate the inflorescence structure in cucumber, yet the mechanisms underlying their responses to environmental factors and the formation of terminal flowers remain elusive. Here, we performed map-based cloning to identify the gene that controls a season-dependent determinate growth phenotype and found that it was caused by the complete deletion of CsTFL1 in the genome of cucumber line WI1983Hde. In the CsTFL1 deletion plants (CsTFL1del), determinate growth could be partially rescued by high-temperature and long-day conditions. The expressions of CsTFL1 and its ortholog CsTFL1d could be upregulated by long-day and high-temperature signals. Knockdown of CsTFL1d resulted in determinate growth and the formation of terminal flowers in WT. These results indicate that the induction of CsTFL1d expression by long-day and high-temperature might partially rescue determinate growth of CsTFL1del. Furthermore, biochemical analyses showed that CsTFL1d interacts directly with CsNOT2a, which indicated that CsTFL1d and CsTFL1 function via similar regulatory mechanism. Our data suggest that CsTFL1 and CsTFL1d co-contribute to inhibit determinate growth by responding to temperature and photoperiod signals. It provides mechanistic insights into how environmental cues sculpt the inflorescence architecture of cucumber.
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